Journal: Cellular and Molecular Life Sciences
Article Title: Recovery of altered neuromuscular junction morphology and muscle function in mdx mice after injury
doi: 10.1007/s00018-014-1663-7
Figure Lengend Snippet: Dystrophin at the neuromuscular junction. Neuromuscular junctions (NMJs) were fluorescently stained with an acetylcholine receptor binding neurotoxin (α-Bungarotoxin, BTX, green), and antibodies against dystropin ( red ). Dystrophin, a stabilizing membrane protein, is present at the NMJ in healthy (wild-type, WT) tissue, but is missing in mdx mice. NMJs from mdx muscle also show drastic morphological differences when compared with NMJs from WT mice. It has been suggested that the lack of dystrophin is indirectly involved with the fragmented appearance of acetylcholine receptors seen in mdx mice. Scale bar equals 10 µm
Article Snippet: We used age-matched male control (WT) and mdx (lacking dystrophin) mice from the C57BL/10ScSnJ strain (The Jackson Laboratory, Bar Harbor, ME).
Techniques: Staining, Binding Assay, Membrane